Deleted
Sample Discontinued
Experience: None
Not Used
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Reporter

Part:BBa_K3176001

Designed by: Tara Fournier   Group: iGEM19_Pasteur_Paris   (2019-10-16)


mRFP for S.aureus with SarA RBS

mRFP1 is a constitutively monomeric red fluorescent protein derived from Discosoma coral drFP583 gene. It is widely used as a reporter because it matures 10 times faster than DsRed and shows a greater tissue penetration. BBa_K3176001 allows the expression of mRFP1 in gram-positive cells. It was designed from BBa_E1010 and adapted for S. aureus and can thus be used as a reporter for this strain by inserting the promoter SarA P1 from BBa_K1323021 and a SarA RBS from BBa_3176010. The excitation peak is at 584 nm and it shows an emission peak at 607 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 623
    Illegal AgeI site found at 735
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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